pcdna3 nano lantern ca2  (Addgene inc)

Journal: Cell Reports Methods

Article Title: Optogenetic induction of hibernation-like state with modified human Opsin4 in mice

doi: 10.1016/j.crmeth.2022.100336

Figure Lengend Snippet: Light stimulation was applied to Qrfp-iCre mouse expressing hOPN4dC-mCherry in the AVPe via an optic fiber (3 μW from the fiber tip, 473-nm, 6 h from time 0). Heart rate and core body temperature are also simultaneously taken by telemetry sensors. Video plays at 60x speed. Images in Figure 1D are created from this video.

Article Snippet: Stimulating light for hOPN4s and excitation light for fluorescence were the same (480 nm, 500 ms, every 2 s), as described in a previous study., For bioluminescence-based Ca2+ recording, cells were transfected with the expression constructs pEF1a-hOPN4s-mCherry and pcDNA3-Nano-lantern(Ca2+)_600 (Addgene, #51982 ).

Techniques:

Journal: Cell Reports Methods

Article Title: Optogenetic induction of hibernation-like state with modified human Opsin4 in mice

doi: 10.1016/j.crmeth.2022.100336

Figure Lengend Snippet: Representative thermal and visual imaging of a Qrfp-iCre mouse expressing hOPN4dC-mCherry in the AVPe, during optogenetic stimulation via a blue LED attached on the skull (250 μW, 1 h from time 0). Core body temperature was simultaneously taken by telemetry system. Video plays at 60x speed. Images in Figure 4B are created from this video.

Article Snippet: Stimulating light for hOPN4s and excitation light for fluorescence were the same (480 nm, 500 ms, every 2 s), as described in a previous study., For bioluminescence-based Ca2+ recording, cells were transfected with the expression constructs pEF1a-hOPN4s-mCherry and pcDNA3-Nano-lantern(Ca2+)_600 (Addgene, #51982 ).

Techniques:

Journal: Cell Reports Methods

Article Title: Optogenetic induction of hibernation-like state with modified human Opsin4 in mice

doi: 10.1016/j.crmeth.2022.100336

Figure Lengend Snippet:

Article Snippet: Plasmid: pcDNA3-Nano-lantern(Ca2+)_600 , Saito et al. (2012) , Addgene #51982.

Techniques: Virus, Recombinant, Transfection, Modification, Cloning, Synthesized, Titration, Plasmid Preparation, Software